The trio debut in "Let's Get Serious" where they show up to defeat the H.I.V.E. Five who were in the middle of a heist. The Teen Titans were also present, but were merely ignored and the three members of Young Justice swiftly and efficiently captured the H.I.V.E. members, catching the eyes of the Titans except for Robin. Aqualad shows his distaste of the way the Teen Titans handle duties and goes on to comment how they aren't serious enough.
The three Young Justice members return once more in the episode where they arrive just after the new "serious Titans" successfully defeated the H.I.V.E. members on their own. Aqualad is impressed on their new seriousness but asks if it's "too serious" to them, earning an upset Cyborg remarking the difficulties of being half machine and human. Robin gathers his serious Titans to take on a new mission uttering the same thing Aqualad said before to Robin that "something tells me this is only the beginning".
In Teen Titans Go!
In Young Justice
- Aqualad (Kaldur'ahm): One of the founding members and the original leader. He left the team to work undercover in the ranks of his father, Black Manta, but once his mission was over he was reinstated as leader by Nightwing.
- Batgirl (Barbara Gordon): Joined the team after 2010.
- Beast Boy (Garfield Logan): Joined the team after 2010.
- Blue Beetle (Jaime Reyes): Joined the team on or after December 1, 2015.
- Bumblebee (Karen Beecher): Joined the team after 2010.
- Guardian (Mal Duncan): He was originally the team's operations manager, coordinating all Squads from the Cave.He became Guardian after Despero's attack on the Hall of Justice.
- Kid Flash: After a failure to return to the future, he joined the team as Impulse in March 2016.
- Lagoon Boy (La'gaan): Joined the team after 2010.
- Miss Martian (M'gann M'orzz): One of the senior members of the team. She has been offered membership of the Justice League, but has turned it down. She still has an open invitation to join the League.
- Tim Drake: Joined the team at the beginning of 2015.
- Static (Virgil Hawkins): Joined the team around July 4, 2016.
- Superboy (Conner Kent): One of the senior members of the team. He has been offered membership of the Justice League, but has turned it down. He still has an open invitation to join the League.
- Tigress (Artemis Crock): Part of the original team, she quit the team to attend college with Kid Flash. She later returned only to fake her death and start working undercover on a new mission as Tigress. After Wally died saving the world, she decided to join the team again, as Tigress.
- Wonder Girl (Cassandra Sandsmark): Joined the team a few months before December 2015.
- Let's Get Serious (debut)
- Justice League's Next Top Talent Idol Star: Second Greatest Team Edition
- Justice League's Next Top Talent Idol Star: Justice League Edition